Diagnostic Salivary 17ß-Estradiol EIA Kit
Product:
Direct Salivary 17ß-Estradiol Enzyme Immunoassay
Intended Use:
Quantitative measurement of salivary Estradiol 17ß
FDA:
IVD, FDA Registered
Catalog Number:
674
Method:
Enzyme Immunoassay (EIA), Colormetric
AMR:
1 pg/mL – 32 pg/mL
LOD:
0.5 pg/mL
Sample Size:
100 µL
Incubation:
2hr @ Room Temperature
# of tests:
96 wells
Reading:
450nm
Storage:
2° - 8°C
Shelf Life:
9 Months
Assay Background:
Assay Principle:
competition principal and microplate separation. An unknown amount of estradiol present in a saliva sample and a fixed amount of estradiol conjugated to horse radish peroxidase (E2- HRP) compete for binding sites with a rabbit monoclonal estradiol antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away. Enzyme substrate solution is then added and a
blue color formed. This reaction is stopped with an acid solution to produce a yellow
color. The optical density is then read at 450 nm. The amount of E2-HRP detected is inversely proportional to the amount of estradiol in a sample.
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