Research Use Serum Melatonin EIA Kit
Product:
Direct Melatonin Serum/Plasma Enzyme Immunoassay
Intended Use:
Quantitative measurement of Melatonin in human serum or plasma
FDA:
RUO
Catalog Number:
534
Method:
Enzyme Immunoassay (EIA), Colormetric
AMR:
3.0 – 300 pg/mL
Sensitivity:
2.5 pg/mL
Sample Size:
25 µL
Incubation:
2hr @ Room Temperature
# of tests:
96 wells
Reading:
450nm
Storage:
2° - 8°C
Shelf Life:
6 Months
Assay Background:
Assay Principle:
The Pantex Direct Melatonin Serum/Plasma Kit, Cat #534 is based on the competition principal and microplate separation. Melatonin in calibrators and samples compete with a fixed amount of melatonin conjugated to horse radish peroxidase (Melatonin-HRP) for binding sites with a rabbit melatonin monoclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm. The amount of Melatonin-HRP detected is inversely proportional to the amount of melatonin in a sample.