Research Use Salivary DHEA EIA Kit
Product:
Specific Salivary DHEA Enzyme Immunoassay
Intended Use:
Quantitative measurement of salivary DHEA
FDA:
RUO
Catalog Number:
626
Method:
Enzyme Immunoassay (EIA), Colormetric
AMR:
10.2 pg/mL– 1000 pg/mL
LOD:
1.1 pg/mL
Sample Size:
50 µL
Incubation:
2hr @ Room Temperature
# of tests:
96 wells
Reading:
450nm
Storage:
2° - 8°C
Shelf Life:
12 Months
Assay Background:
Assay Principle:
The Pantex Salivary Dehydroepiandrosterone (DHEA) EIA kit, Cat #626 is based on the competition principal and microplate separation. DHEA calibrators of known concentration and unknown amounts of DHEA in saliva samples compete with a fixed amount of DHEA conjugated to horse radish peroxidase (DHEA-HRP) for binding sites with a rabbit DHEA polyclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm. The amount of DHEA-HRP detected is inversely proportional to the amount of DHEA in a sample.