Research Use Salivary DHEA EIA Kit
Specific Salivary DHEA Enzyme Immunoassay
Quantitative measurement of salivary DHEA.
Enzyme Immunoassay (EIA), Colormetric
Analytical Measurement Range:
10.2 pg/mL– 1000 pg/mL
Limit of Detection:
2hr @ Room Temperature
# of tests:
2° - 8°C
The Pantex Salivary Dehydroepiandrosterone (DHEA) EIA kit, Cat #626 is based on the competition principal and microplate separation. DHEA calibrators of known concentration and unknown amounts of DHEA in saliva samples compete with a fixed amount of DHEA conjugated to horse radish peroxidase (DHEA-HRP) for binding sites with a rabbit DHEA polyclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm. The amount of DHEA-HRP detected is inversely proportional to the amount of DHEA in a sample.