Research Use Salivary Free Estriol(uE3) EIA Kit
Product:
Salivary Unconjugated (Free) Estriol Enzyme Immunoassay
Intended Use:
Quantitative measurement of salivary Free Estriol
FDA:
RUO
Catalog Number:
633
Method:
Enzyme Immunoassay (EIA), Colormetric
Analytical Measurement Range:
5.0 pg/mL–1,215 pg/mL
Limit of Detection:
0.8 pg/mL
Sample Size:
50 µL
Incubation:
2hr @ Room Temperature
# of tests:
96 wells
Reading:
450nm
Storage:
2° - 8°C
Shelf Life:
12 Months
Assay Background:
Assay Principle:
The Pantex Salivary Free Estriol EIA kit, Cat #633, is based on the competition principal and microplate separation. Estriol calibrators and unknown amounts of estriol in saliva samples compete with a fixed amount of estriol conjugated to horse radish peroxidase (Estriol-HRP) for binding sites with a rabbit estriol monoclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm. The amount of Estriol-HRP detected is inversely proportional to the amount of estriol in a sample.