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Research Use Salivary Free Estriol(uE3) EIA Kit

Product:

Salivary Unconjugated (Free) Estriol Enzyme Immunoassay

Intended Use:

Quantitative measurement of salivary Free Estriol

FDA:

RUO

Catalog Number:

633

Method:

Enzyme Immunoassay (EIA), Colormetric

Analytical Measurement Range:

5.0 pg/mL–1,215 pg/mL

Limit of Detection:

0.8 pg/mL

Sample Size: 

50 µL

Incubation: 

2hr @ Room Temperature

# of tests: 

96 wells

Reading: 

450nm

Storage:

2° - 8°C 

Shelf Life: 

12 Months

Assay Background:


Estriol is the weakest of the three major estrogens with a binding affinity for the estrogen receptor about 1/100 that of estradiol. Physiologically significant amounts of Estriol are only present during pregnancy or with supplementation. Estriol is available, mainly, as a custom compounded product and is one of the components of bi- or tri-estrogen creams usually in a ratio of estradiol to estrone to estriol 1:1:8. Salivary estriol median levels are <2 pg/ml in non- pregnant pre- and post-menopausal women as well as in men. These levels represent little to no physiologic estrogenic activity. In pregnancy, low levels of Estriol may indicate fetal distress and in Down Syndrome Estriol measurements are part of a screen for this condition.




Assay Principle:


The Pantex Salivary Free Estriol EIA kit, Cat #633, is based on the competition principal and microplate separation. Estriol calibrators and unknown amounts of estriol in saliva samples compete with a fixed amount of estriol conjugated to horse radish peroxidase (Estriol-HRP) for binding sites with a rabbit estriol monoclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm. The amount of Estriol-HRP detected is inversely proportional to the amount of estriol in a sample.




Assay Protocol


Click here to view





Related Products:

Monoclonal

Antibody

Anti-Estriol-C-6

Polyclonal

Antibody

Anti-Estriol-C-6

Matching

HRP

Estriol-6-CMO-HRP

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